ABSTRACT
Objective: To develop an HPLC-DAD method for the detemination of the content change of honokiol and magnolol from classical Pingweisan (a prescription of Chinese materia medica used for stomach discomfort), and it is based on minister (Chen) medicine compatibility. Methods: After the decomposition of classical Pingweisan besed on Magnoliae Officinalis Cortex as minister medicine, 11 groups (one individual herb group, four compound groups, six groups with Magnoliae Officinalis Cortex as main medicine) were divided. The analysis was carried out on a GRACE AlltimaTM C18 (250 mm × 4.6 mm, 5 μm) column with a mobile phase which was composed of acetonitrile (B) and water (A) for isocratic elution. The column temperature was 25 ℃, the flow rate was 1 mL/min, and the detection wavelength was set at 294 nm. Results: The linear relationship of honokiol and magnolol is better within the ranges in 20.12-120.72 μg/mL (r = 0.999 0) and 20.16-120.96 μg/mL (r = 0.999 1); Study on the precision and repeatability, the RSD value of both honokiol and magnolol was less than 2%, which showed that the precision of the instrument and the method of repeatability were favourable. Stability test showed that the determination of the components was stable within 24 h. The recoveries were 98.69% and 101.28%, and the RSD values were 1.47% and 1.24%, which showed that the method had a good accuracy. Monarch Atractylodis Rhizoma, Zingberis, and Jujubae Fructus could make the contents of honokiol and magnolol in Pingweisan reduced; while the assistant Citri Reticulatae Pericarpium and guiding Glycyrrhizae Radix could make the contents increased. Conclusion: The other crude drugs in Pingweisan could affect and change the contents of honokiol and magnolol. It could explain the synergistic effect of compatibility and detoxification of the classical compound Pingweisan.
ABSTRACT
<p><b>BACKGROUND</b>Copernicus optical coherence tomography (SOCT) is a new, ultra high-speed and high-resolution instrument available for clinical evaluation of optic nerve. The purpose of the study was to compare the agreements between SOCT and Heidelberg retinal tomography (HRT).</p><p><b>METHODS</b>A total of 44 healthy normal volunteers were recruited in this study. One eye in each subject was selected randomly. Agreement between SOCT and HRT-3 in measuring optic disc area was assessed using Bland-Altman plots. Relationships between measurements of optic nerve head parameter obtained by SOCT and HRT-3 were assessed by Pearson correlation.</p><p><b>RESULTS</b>There was no significant difference in the average cup area (0.306 vs. 0.355 mm, P = 0.766), cup volume (0.158 vs. 0.130 mm, P = 0.106) and cup/disc ration (0.394 vs. 0.349 mm, P = 0.576) measured by the two instruments. However, other optic disc parameters from SOCT were significantly lower compared with HRT-3. The Bland-Altman plot revealed good agreement of cup area and cup volume measured by SOCT and HRT-3. Bad agreement of disc area, rim area, rim volume and cup/disc ratio were found between SOCT and HRT-3. The highest correlations between the two instruments were observed for cup area (r(2) = 0.783, P = 0.000) and cup/disc ratio (r(2) = 0.669, P = 0.000), whereas the lowest correlation was observed for disc area (r(2) = 0.100, P = 0.037), rim area (r(2) = 0.275, P = 0.000), cup volume (r(2) = 0.005, P = 0.391) and rim volume (r(2) = 0.021, P = 0.346).</p><p><b>CONCLUSIONS</b>There were poor agreements between SOCT and HRT-3 for measurement of optic nerve parameters except cup area and cup volume. Measurement results of the two instruments are not interchangeable.</p>